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Wednesday, July 22, 2020 | History

2 edition of role of erk and intracellular RHAMMv4 on microtubule stability in transformation. found in the catalog.

role of erk and intracellular RHAMMv4 on microtubule stability in transformation.

Rene E. Harrison

role of erk and intracellular RHAMMv4 on microtubule stability in transformation.

by Rene E. Harrison

  • 207 Want to read
  • 31 Currently reading

Published .
Written in English


The Physical Object
Pagination171 leaves.
Number of Pages171
ID Numbers
Open LibraryOL20385420M
ISBN 10061253653X

The microtubule-associated protein tau is known for its ability to bind to and stabilize microtubules and for its ability to nucleate microtubule assembly. In neurodegenerative tauopathies such as Alzheimer's disease, tau becomes hyperphosphorylated and loses the capacity for microtubule binding, possibly. γ-tubulin organizes capped microtubule ends at the centrosome periphery. To investigate the role of γ-tubulin on mitotic centrosome organization, we first visualized the distribution of α-tubulin and γ-tubulin on immunostained wild-type and tbg-1(RNAi) embryos ().A linescan through a wild-type centrosome revealed a clear reduction in α-tubulin levels within the core region.

  Tau is one of the microtubules associated proteins (MAP) that is predominantly localized in the axons in the developed neurons and is thought to play important role in the maintenance of microtubule stability. In the human brain, there are six isoforms of : Hasan A.M.M. Almansoub, Hasan A.M.M. Almansoub, Hui Tang, Ying Wu, Ding-Qi Wang, Yacoubou Abdoul Raz. Start studying Combo with "SB Book 1" and 3 others. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Cell Culture and Transfection. LLC-PK1α (pig kidney epithelial) cells, stably transfected with green fluorescent protein (GFP)-α-tubulin (Rusan et al., ) were plated at low density (∼ cells/mm 2) on mm glass-bottomed dishes (MatTek, Ashland, MA) and allowed to spread overnight at 37°C and 5% CO 2 in Opti-MEM I (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine by: Mitotic catastrophe (MC) has long been accounted as a cell death path activated by premature or inappropriate entry of cells into mitosis following chemical or physical stresses. Although various possible explanations related to MC have been formulated, no general accepted definition of this phenomenon has been found yet. Recent evidences, however, demonstrate that MC is not a distinguished Cited by: 3.


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Role of erk and intracellular RHAMMv4 on microtubule stability in transformation by Rene E. Harrison Download PDF EPUB FB2

Several types of cancer. RHAMMv4 is a key regulator of the ras pathway and enhances erk activation in murine fibroblasts. ln vitro studies have previously shown that microtubulc (MT)-associated proteins (iMAPs) and MT catastrophe factors are potential cytoplasmic targets of erk.

Erk-mediated phosphorylation of iMAPs has been reported to rcduce their ability to stabilize MTs in : Rene E. Harrison. The effect of erk kinases on MT stability could contribute to the documented role of these kinases in regulating cell motility, invasion, and proliferation.

MTs serve as tracks for cellular transport and regulate cell shape, polarity, and cell process formation, all of which are required functions for cell motility [ 4 ].Cited by: Active Erk Regulates Microtubule Stability in H-ras Erk, ras oncogene, microtubule stability, breast epithelia fibroblast.

the role of erk kinases in these additional cellular compart. Because of the role of MTs in proliferative, motile, and invasive processes [6–9], and recent studies linking erk and its upstream activator kinase MKK1 [27–29] to breast cancer progression, we have assessed the role of the ras– erk cascade in regulating MT stability both in Cited by: The downside of microtubule stability.

The role of erk and intracellular RHAMM on microtubule stability in transformation [microform]. The pattern of expression of the microtubule-binding protein RHAMM/IHABP in mammary carcinoma suggests a role in the invasive behaviour of Cited by: RHAMM protein forms co-associate with erk kinase The presence of the erk phosphoacceptor sites, the ability of erk I kinase to phosphorylated recombinant RHAMM in vitro (unpublished data), reports that RHAMM regulates erk activation l6,17 and the co-immunoprecipitation of erk 1 kinase'" with RHAMM suggest that one function of intracellular Author: R.

Harrison, F.-S. Wang, E.A. Turley. Activation of extracellular signal-regulated kinase (ERK) 1/2 mitogen-activated protein kinase (MAPK) occurs during cardiomyocyte isolation and may impact on microtubule stability. Retinoic acids in turn could accelerate ERK1/2 deactivation by enhancing expression of MAPK phosphatases (23).Cited by: We previously demonstrated that microtubule disruption impairs stimulation of glucose uptake in cardiomyocytes and that 9-cis retinoic acid (9cRA) treatment preserved both microtubule integrity and Cited by: Under physiological conditions, tau binds to and stabilizes existing microtubules and promotes microtubule growth, but pathological hyperphosphorylation leads to self-assembly of tau, which results both in tau tangles and also in microtubule detabilization.

In contrast, the role of phosphorylation on stathmin activity is much better by: 2. Fig. ERK phosphorylation in vitro. (A) Schematic representation of the input-output response of ERK MAP distributive phosphorylation model leads to switch-like response.

On the other hand, no model is currently validated for the graded response of ERK, which is observed in. What is Phospho ERK. Rene E.

Harrison has written: 'The role of erk and intracellular RHAMMv4 on microtubule stability in transformation' Load More. Researchers determined that microtubules, a type of molecular “strut,” play a key role in regulating contractile function in mouse and rat heart cells.

Microtubule alterations that impair contraction were found in diseased human hearts, suggesting that modifying microtubules may help restore heart function. Cytoskeletal proteins, which consist of different sub-families of proteins including microtubules, actin and intermediate filaments, are essential for survival and cellular processes in both normal as well as cancer cells.

However, in cancer cells, these mechanisms can be altered to promote tumour development and progression, whereby the functions of cytoskeletal proteins are co-opted to Author: Mei Shan Ong, Shuo Deng, Clarissa Esmeralda Halim, Wanpei Cai, Tuan Zea Tan, Ruby Yun-Ju Huang, Gaut.

We have not yet compared microtubule stability in cells adhered by WT and the YA mutant integrin. This is an important future direction. Integrins regulate a number of other signaling proteins that target microtubule dynamics, such as the Rho family of GTPases, which influence microtubule growth and stability (50–56).Cited by:   Protein transport plays a critical role in the interaction of the cell with its environment.

Recent studies have identified TSC1 and TSC2, two tumor suppressor genes involved in tuberous sclerosis complex, as regulators of the mammalian target of rapamycin (mTOR) pathway.

Cells deficient in TSC1 or TSC2 possess high levels of Rheb-GTP resulting in constitutive mTOR by: Based on these observations, the mechanical role for microtubules in terms of cell tensegrity needs to be further refined.

Moreover, the 3D matrix stiffness alters the microtubule stability and subsequently downstream signaling such as the regulation of Rho, however, the mechanisms still remain not fully understood (Heck et alMyers et al.

A Novel Role for Copper in Ras/Mitogen-Activated Protein Kinase Signaling Michelle L. Turski,a* Donita C. Brady,a Hyung J. Kim,b Byung-Eun Kim,a Yasuhiro Nose,a Christopher M. Counter,a Dennis R.

Winge,c and Dennis J. Thielea Department of Pharmacology and Cancer Biology, Duke University Medical School, Durham, North Carolina, USAa; Departments of Medicine and Biochemistry. Active Erk Regulates Microtubule Stability in H-ras–Transformed Cells1 stability and turnover of MTs during the transformation process.

Microtubule-associated proteins (MAPs), such as tau and MAP-1, bind directly to tubulin and are members of a the role of erk. Protein Expression and Purification. βARK and GRK5 were overexpressed in and purified from Sf9 insect cells (20, 21).Purified rhodopsin kinase was generously provided by Drs.

Pitcher and R. Lefkowitz, and purified Gβ 1 γ 2 was generously provided by Dr. Kennedy. Partially purified tubulin (PPT) containing microtubule-associated proteins was prepared from porcine brain by one to. The invasion of cancer cells into surrounding tissue and the vasculature is essential for tumor metastasis.

Increasing evidence indicates that hepatocyte growth factor (HGF) induces cancer cell migration and invasion.

A broad spectrum of mechanisms underlies cancer cell migration and invasion. Cytoskeletal reorganization is of central importance in the development of the phenotype of cancer Cited by:   Physiological role for Ctr1 in Erk activation in mice.

The results presented here demonstrate a strong role for Cu in the Ras/Raf/Mek/Erk signal transduction pathway in flies and cultured cells and support direct Cu binding to Mek1.

Moreover, our results suggest that a Cu transport-competent form of Ctr1 is required to provide Cu to Mek1.The role of the cytoskeleton in intracellular transport In Section 3 we explained how vesicles bud from donor membranes and fuse with target membranes, which may be quite distant.

In such cases, vesicles are actively transported from one site to another, a process that involves motor proteins attached to the vesicle, which propel the.